Fluorescence resonance energy transfer detection methods: Sensitized emission and acceptor bleaching
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چکیده
منابع مشابه
Fluorescence resonance energy transfer detection methods: Sensitized emission and acceptor bleaching
The present study compared the advantages and disadvantages of fluorescence resonance energy transfer (FRET) determination technologies, namely, sensitized emission (SE) and acceptor bleaching (AB), in order to analyze the applicability of SE and AB for studies investigating particularly interesting new cysteine histidine-rich protein 1 (PINCH1)/integrin-linked kinase (ILK) interaction. HeLa ce...
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Fluorescence resonance energy transfer (FRET) is an extremely effective tool to detect molecular interaction at suboptical resolutions. One of the techniques for measuring FRET is acceptor photobleaching: the increase in donor fluorescence after complete acceptor photobleaching is a measure of the FRET efficiency. However, in wide-field microscopy, complete acceptor photobleaching is difficult ...
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ژورنال
عنوان ژورنال: Experimental and Therapeutic Medicine
سال: 2014
ISSN: 1792-0981,1792-1015
DOI: 10.3892/etm.2014.1928